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KMID : 0613820190290101164
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Journal of Life Science
2019 Volume.29 No. 10 p.1164 ~ p.1170
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A Possible Protective Role of Ginko biloba Outer Seed Coat Methanol Extracts on DNA Damage Induced by H2O2 in HaCaT Human Skin Keratinocytes
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Sim Jae-Young
Lee Jong-Hwan
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Abstract
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The present study was carried out to evaluate extracts of Ginko biloba’s outer seed coat, their antioxidative effects, and their ability to protect against DNA damage due to hydrogen peroxide (H2O2) treatments in cultured human keratinocyte (HaCaT) cells. The bioassays applied for determining the antioxidant effects of a G. biloba outer seed coat water extract (GOSWE) and a G. biloba outer seed coat methanol extract (GOSME) included the DPPH and H2O2 radical scavenging assays. Our results revealed that GOSME had higher activity than GOSWE against H2O2 radical scavenging activity in in vitro and in vivo bioassays. Treatment with GOSME significantly increased the viability of H2O2-treated HaCaT cells. GOSME’s ability to protect against DNA damage was observed via the analysis of plasmids in vitro and genomic DNA in H2O2-treated HaCaT cells. According to our data, GOSME is able to protect HaCaT cells from H2O2-induced DNA damage and apoptosis by blocking cellular damage related to oxidative stress. In conclusion, our study indicated GOSME might serve as a novel agent for the treatment and prevention of skin disorders caused by oxidative stress.
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KEYWORD
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DNA damage, GOSME, GOSWE, H2O2, ROS
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